Selective Separation and Analysis of Catecholamines in Urine Based on Magnetic Solid Phase Extraction by Mercaptophenylboronic Acid Functionalized Fe3O4-NH2@Au Magnetic Nanoparticles Coupled with HPLC

A novel magnetic solid phase extraction based on mercaptophenylboronic acid (MPBA)-functionalized Fe3O4-NH2@Au nanomaterial (Fe3O4-NH2@Au-MPBA) was developed for selective separation and enrichment of catecholamines (including dopamine, norepinephrine adrenaline). Fe3O4-NH2@Au-MPBA nanoparticles were achieved by self-assembly-anchoring MPBA molecules the surface nanocomposites, which synthesized via a facial ultrasonic auxiliary in situ reduction process. The interaction between cis-diol from boronic reversible could be flexibly controlled adjusting pH value. quickly adsorbed weak alkaline solution (pH 8.0–9.0) subsequently released 1.0–2.0). process adsorption dissociation very fast. Furthermore, three detected urine children high performance liquid chromatography (HPLC) with electrochemical detector. Under optimal conditions, (NE), epinephrine (EP) dopamine (DA) separated well internal standard exhibited good linearity range 2.5–500.0 ng mL?1, correlation coefficients r2 > 0.9907. Limits detection (LOD) (signal to noise = 3) 0.39, 0.27 0.60 mL?1 NE, EP DA, respectively. Recoveries spiked 85.4–105.2% relative deviation (RSD) < 11.5%.